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mouse anti myosin7a  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti myosin7a
    Mouse Anti Myosin7a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin7a+mouse/anti-Unconventional+myosin-VIIa/bio_rxiv__2025__07__11__664127-76-41-45
    Average 95 stars, based on 48 article reviews
    mouse anti myosin7a - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Gata3 is required in late proneurosensory development for proper sensory cell formation and organization.
    Article Snippet: The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4 °C for three nights.The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4 °C for three nights.. The following primary antibodies were used: MYO6 Rabbit (Sigma; 1:1000), MYOSIN7A Mouse (DSHB; 1:200), MYSOIN7A Rabbit (Proteus Biosciences, Inc.; 1:500), Neurofilament 200 (NF200) HC Chicken (Aves; 1:200), and SOX2 Rabbit (Sigma; 1:500).. Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4 °C in secondary antibody in blocking buffer.Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4 °C in secondary antibody in blocking buffer.

    Article Title: Gata3 is required in late proneurosensory development for proper sensory cell formation and organization
    Article Snippet: The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4 °C for three nights.The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4 °C for three nights.. The following primary antibodies were used: MYO6 Rabbit (Sigma; 1:1000), MYOSIN7A Mouse (DSHB; 1:200), MYSOIN7A Rabbit (Proteus Biosciences, Inc.; 1:500), Neurofilament 200 (NF200) HC Chicken (Aves; 1:200), and SOX2 Rabbit (Sigma; 1:500).. Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4 °C in secondary antibody in blocking buffer.Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4 °C in secondary antibody in blocking buffer.

    Article Title: Gata3 is Required in Late Proneurosensory Development for Proper Sensory Cell Formation and Organization
    Article Snippet: The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4°C for three nights.The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4°C for three nights.. The following primary antibodies were used: MYO6 Rabbit (Sigma; 1:1000), MYOSIN7A Mouse (DSHB; 1:200), MYSOINVIIA Rabbit (Proteus Biosciences, Inc.; 1:500), Neurofilament 200 HC Chicken (Aves; 1:200), PROX1 Goat (R & D Systems; 1:200), and SOX2 Rabbit (Sigma; 1:500).. Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4°C in secondary antibody in blocking buffer.Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4°C in secondary antibody in blocking buffer.

    Mutagenesis:

    Article Title: Gata3 is required in late proneurosensory development for proper sensory cell formation and organization.
    Article Snippet: The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4 °C for three nights.The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4 °C for three nights.. The following primary antibodies were used: MYO6 Rabbit (Sigma; 1:1000), MYOSIN7A Mouse (DSHB; 1:200), MYSOIN7A Rabbit (Proteus Biosciences, Inc.; 1:500), Neurofilament 200 (NF200) HC Chicken (Aves; 1:200), and SOX2 Rabbit (Sigma; 1:500).. Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4 °C in secondary antibody in blocking buffer.Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4 °C in secondary antibody in blocking buffer.

    Article Title: Gata3 is required in late proneurosensory development for proper sensory cell formation and organization
    Article Snippet: The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4 °C for three nights.The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4 °C for three nights.. The following primary antibodies were used: MYO6 Rabbit (Sigma; 1:1000), MYOSIN7A Mouse (DSHB; 1:200), MYSOIN7A Rabbit (Proteus Biosciences, Inc.; 1:500), Neurofilament 200 (NF200) HC Chicken (Aves; 1:200), and SOX2 Rabbit (Sigma; 1:500).. Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4 °C in secondary antibody in blocking buffer.Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4 °C in secondary antibody in blocking buffer.

    Article Title: Gata3 is Required in Late Proneurosensory Development for Proper Sensory Cell Formation and Organization
    Article Snippet: The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4°C for three nights.The tissue was incubated in primary antibodies, diluted in blocking buffer, at 4°C for three nights.. The following primary antibodies were used: MYO6 Rabbit (Sigma; 1:1000), MYOSIN7A Mouse (DSHB; 1:200), MYSOINVIIA Rabbit (Proteus Biosciences, Inc.; 1:500), Neurofilament 200 HC Chicken (Aves; 1:200), PROX1 Goat (R & D Systems; 1:200), and SOX2 Rabbit (Sigma; 1:500).. Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4°C in secondary antibody in blocking buffer.Next, the tissue was washed four times thirty minutes, followed by overnight incubation at 4°C in secondary antibody in blocking buffer.



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    Developmental Studies Hybridoma Bank mouse anti myosin7a
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    KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. <t>Myosin7a</t> antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.
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    KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. <t>Myosin7a</t> antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.
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    KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. <t>Myosin7a</t> antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.
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    KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. <t>Myosin7a</t> antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.
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    KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. <t>Myosin7a</t> antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.
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    Developmental Studies Hybridoma Bank mouse anti-myosin7a
    KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. <t>Myosin7a</t> antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.
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    Image Search Results


    KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. Myosin7a antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.

    Journal: iScience

    Article Title: mTORC2 regulates auditory hair cell structure and function

    doi: 10.1016/j.isci.2023.107687

    Figure Lengend Snippet: KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. Myosin7a antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.

    Article Snippet: Mouse monoclonal anti-myosin7a IgG1 , Developmental Studies Hybridoma Bank , Cat#138-1s; RRID: AB_2282417.

    Techniques: Negative Control, Positive Control, Staining, Fluorescence

    Hearing loss precedes hair cell loss in HC-RicKO mice (A) Cochleograms showing inner hair cell (left graphs) and outer hair cell (right graphs) loss in 5 percent (%) distances from the Apex at indicated timepoints. Place-frequency map calculated with formula d = (LOG10((f+6.664)/9.8)/LOG10(10))/0.0092 (where d is the distance from the Apex in % and f the frequency in kHz). , , n = 3–4 mice (2 weeks), 4 mice (4 weeks), 3 mice (8 weeks), and 3–4 mice (12 weeks) per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (B) Representative images (maximum intensity projections) of the medial cochlear turn from 8-week-old mice of both genotypes. Hair cells are visualized with phalloidin, a Myosin7a antibody and nuclear DAPI staining. Scale bar for all figures = 20 μm.

    Journal: iScience

    Article Title: mTORC2 regulates auditory hair cell structure and function

    doi: 10.1016/j.isci.2023.107687

    Figure Lengend Snippet: Hearing loss precedes hair cell loss in HC-RicKO mice (A) Cochleograms showing inner hair cell (left graphs) and outer hair cell (right graphs) loss in 5 percent (%) distances from the Apex at indicated timepoints. Place-frequency map calculated with formula d = (LOG10((f+6.664)/9.8)/LOG10(10))/0.0092 (where d is the distance from the Apex in % and f the frequency in kHz). , , n = 3–4 mice (2 weeks), 4 mice (4 weeks), 3 mice (8 weeks), and 3–4 mice (12 weeks) per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (B) Representative images (maximum intensity projections) of the medial cochlear turn from 8-week-old mice of both genotypes. Hair cells are visualized with phalloidin, a Myosin7a antibody and nuclear DAPI staining. Scale bar for all figures = 20 μm.

    Article Snippet: Mouse monoclonal anti-myosin7a IgG1 , Developmental Studies Hybridoma Bank , Cat#138-1s; RRID: AB_2282417.

    Techniques: Staining

    Journal: iScience

    Article Title: mTORC2 regulates auditory hair cell structure and function

    doi: 10.1016/j.isci.2023.107687

    Figure Lengend Snippet:

    Article Snippet: Mouse monoclonal anti-myosin7a IgG1 , Developmental Studies Hybridoma Bank , Cat#138-1s; RRID: AB_2282417.

    Techniques: Recombinant, Saline, Electron Microscopy, Software, Imaging